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KMID : 1134820090380070832
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Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 7 p.832 ~ p.838
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Effects of Solvent Extracts from Dried Beet (Beta vulgaris) on Antioxidant in Cell Systems and Growth of Human Cancer Cell Lines
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Jang Joo-Ri
Kim Kyung-Kun
Lim Sun-Young
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Abstract
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The inhibitory effects of solvent extracts from dried beet (Beta vulgaris) on H₂O₂-induced oxidative stress in cell systems and on the growth of cancer cell lines (HT-29 human colon cancer and AGS human gastric adenocarcinoma cells) were investigated. Inhibitory effects of acetone with methylene chloride (A＋M) and methanol (MeOH) extracts on the growth of HT-29 and AGS cancer cells increased in a dose dependent manner (p＜0.05). The inhibitory effect was more significant on the growth of AGS cells and A＋M extracts had a higher inhibitory effect compared to MeOH extracts. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of both cancer cells (p＜0.05). Among fractions, hexane and 85% aq. methanol fractions showed higher inhibitory effects. In order to determine the protective effect on H₂O₂-induced oxidative stress, DCHF-DA (dichlorodihydrofluorescin diacetate) assay was conducted. The A＋M and MeOH extracts of dried beet appeared to significantly reduce the levels of intracellular (ROS) with dose responses. Among the fractions, 85% methanol fractions showed a higher protective effect on production of lipid peroxides. These results indicate that the intake of dried beet may improve oxidative stress in cell and reduce cancer risk.
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KEYWORD
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dried Beta vulgaris, human cancer cell, growth inhibition, antioxidant, reactive oxygen species
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